Biologia, Bratislava 55/3: 283-287, 2000.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full Paper

Isolation, identification and determination of insecticidal activity of two insect-originated Bacillus spp.


Mustafa Yaman & Zihni Demirbag*

Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University, 61080-Trabzon, Turkey; tel: 90-462-325 3200, fax: 90-462-325 3195, e-mail:

* corresponding author

Received: August 5, 1999 / Accepted: February 16, 2000



Two species of Bacillus, B. thuringiensis and B. sphaericus were isolated from Hyphantria cunea and Pieris brassicae, respectively. These bacteria were identified and tested as a biological control agents against the hazelnut pests, Gypsonoma dealbana, Euproctis chrysorrhoea, Melolontha melolontha and Agelastica alni. B. thuringiensis and B. sphaericus have 93 and 29% insecticidal activities on G. dealbana, 58 and 37% on E. chrysorrhoea, 44 and 32% on M. melolontha and 24 and 38% on A. alni respectively.


Key words: B. thuringiensis, B. sphaericus, biological control, hazelnut pests.