Biologia, Bratislava 55/3: 259-265, 2000.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full Paper

Accumulation of two cytoplasmic polypeptides in roots of Al-sensitive and Al-resistant barley cultivars during Al, pH and metal stress.

 

Ladislav Tamas*, Jana Huttova, Igor Mistrik & Lenka Hajasova

Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 14, SK-84223 Bratislava, Slovakia; tel.: ++421-7-59412686, fax: ++421-7-54771948, e-mail: botutama@savba.savba.sk

* corresponding author

Received: August 25, 1999 / Accepted: February 16, 2000

 

Abstract

The accumulation of two cytoplasmic polypeptides (27.5 and 28.0 kDa) in roots of Al-sensitive (Alfor) and Al-resistant (Bavaria) barley cultivars were analysed during stress conditions induced by different Al3+ concentrations, pH values of root medium, and some heavy metals (Cu2+, Cd2+, Co2+) treatment. In both cultivars aluminium (Al3+) in concentrations ranging from 5 up to 150 ÁM induced an increased accumulation of 27.5 and 28 kDa cytoplasmic polypeptides. In Al-resistant barley this accumulation appeared very early after the application of AlCl3 into the root medium, while in Al-sensitive cultivar 24 h delay was observed. Increased concentrations of Al in root solution from 10 up to 150 ÁM did not cause quantitative differences in polypeptide pattern of root cytoplasmic proteins between the two barley cultivars. Changes in pH values of root medium (pH 3.5-6.5) did not show any effect on the accumulation of 27.5 and 28 kDa polypeptides in Al-sensitive or in Al-tolerant barley cultivar. However, differences were found between the two barley cultivars during heavy metal treatment. Application of 10 ÁM concentration of heavy metals (Cu, Cd, and Co) in the root medium on Al-sensitive cultivar Alfor resulted in similar accumulation of 27.5 and 28.0 kDa polypeptides as it was found after Al treatment. On the other hand, the metal stress did not induce accumulation of these two polypeptides in roots of Al-resistant cultivar Bavaria. Our data suggest that 27.5 and 28.0 kDa polypeptides are not specifically involved in Al resistance. These polypeptides probably belong to the group of non specific stress polypeptides which accumulate in roots of Al-sensitive barley during Al and heavy metal treatment, while in Al-resistant cultivar only during Al treatment.

 

Key words: Hordeum vulgare L., barley, Al, Cu, Cd, Co, cytoplasmic proteins, roots, stress proteins.