Biologia, Bratislava 55/3: 243-247, 2000.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full Paper

Factors affecting the production of pyrenocine A  by Pyrenochaeta sp.


Katarina Lesova1*, Maria Sturdikova1 & Bohumil Proksa2

1 Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Technical University, Radlinskeho 9, SK-81237 Bratislava, Slovakia; tel: ++421 7 59325 712, fax: ++421 7 367085, e-mail:

2 Slovakofarma a. s., SK-92027 Hlohovec, Slovakia

* corresponding author

Received: November 16, 1999 / Accepted: February 16, 2000



Factors affecting the production of antibiotic pyrenocine A  by Pyrenochaeta sp. in a shaken culture were studied. The effect of carbon and nitrogen sources on production of the aforementioned metabolite was established; its highest production  (27.8 mg/L) was observed with a medium containing both maltose and peanut flour in concentrations 80 g/L and 16 g/L, respectively. Concentration of inorganic salts (NaNO3, KH2PO4 , KCl, MgSO4.7H2O, FeSO4.7H2O) in culture medium also affected  production of pyrenocine A. Increasing their concentration lowered the production of the studied metabolite. Therefore the highest production of pyrenocine A (29.2 mg/L) was observed in a medium without external addition of inorganic salts. On the other hand the growth of the culture was proportionally enhanced with increasing concentration of salts in medium. 


Key words: Pyrenochaeta, pyrenocine A, antifungal antibiotic, production.