Biologia, Bratislava, 56/Suppl. 9: 91-95, 2001.
ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).
Maria Stara*, Shubhada Bopegamage & Anna Petrovicova
Institute of Preventive and Clinical Medicine, Limbova 14, SK-83301 Bratislava, Slovakia; fax: ++ 421 7 54773906, e-mail: firstname.lastname@example.org
* corresponding author
Received: January 19, 2001 / Accepted: May 18, 2001
Compelling data have been accumulated recently on the participation of coxsackieviral infection (namely type B3 and B4) in the aetiology and pathogenesis of some serious diseases like myocarditis, pancreatitis, diabetes mellitus and others. In this part of the experimental study we followed up the course of humoral immune response in mice perorally infected with coxsackie viruses B3 (strain Nancy) and B4 (strain JVB), respectively. The sera collected from infected and control animals in 18 different intervals (up to 96 day post infection) were tested for the presence of anti-coxsackieviral antibodies by in-house Western-blot method. The positive reaction was observed on the day 4 in mice infected with coxsackie B3 and on day 6 in mice infected with coxsackie B4 viruses. The results were comparable to those obtained in virus-neutralisation tests. Our results show that oral route as the natural way of coxsackievirus infection can be successfully used also in pathogenetical studies. We also proved that Western-blot, the simple and rapid serological method can be successfully used for antibody detection in diagnostic of coxsackieviral infection.
Key words: viruses coxsackie, experimental peroral infection, humoral immune response, Western-blot.