Biologia, Bratislava, 56/Suppl. 9: 69-73, 2001.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full Paper

Detection and antibiotic resistance of Shiga toxin gene positive E. coli isolated from rectal swabs of children with diarrhoea in Slovakia.

 

Marian Sabol1*, Leonard Siegfried1, Adriana Liptakova1, Marta Kmetova1, Hedviga Sehnalkova2, Ludmila Podracka3 & Daniela Kotulova4

1 Institute of Medical Microbiology, Faculty of Medicine, P.J. Safarik University, Srobarova 2, SK-04180 Kosice, Slovakia; phone: ++ 421 95 6225471, fax: ++ 421 95 6420253, e- mail: mfsabol@central.medic.upjs.sk

2 Department of Microbiology, Faculty of Medicine, P.J. Safarik University, Srobarova 2, SK-04180 Kosice, Slovakia

3 Pediatric Clinic, University Hospital, Tr. SNP 1, SK-04167 Kosice, Slovakia

4 Institute of Microbiology, Medical Faculty, Comenius University, Sasinkova 4, SK-81434 Bratislava, Slovakia

* corresponding author

Received: February 9, 2001 / Accepted: May 7, 2001

 

Abstract

Infection with Shiga toxin-producing Escherichia coli (STEC) is not only sporadic, but also associated with outbreaks with hundreds of infected persons.  The main virulence factors of STEC are Shiga toxins. These cytotoxins cause the principal symptoms in patients. The onset of manifestations is rather nonspecific and could mask real pathogen, the STEC. Rapid diagnosis of STEC is especially  important to prevent fatal complications and death in risk groups. There were only sporadic cases of hemolytic uremic syndroms, which could be related to STEC in Slovakia.  We decided to contribute to the detection of STEC and here we present our experiences with fundamental assays for identification of STEC.  Altogether, we assayed 70 faecal samples, seven of which were PCR positive for stx. None of them produced toxins by Vero cell assay and Oxoid agglutination test.  There was good concordance between all used tests with clinical samples and control strains. We also have found out that the isolates with stx genes were sensitive to majority of used antibiotics and resistance was linked with ampicilin, amoxicillin/clavulanic acid, and spiramycin.

 

Key words: Shiga toxins, STEC, antibiotics, resistance, Slovakia.