Biologia, Bratislava, 56/Suppl. 9: 65-68, 2001.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full Paper

Detection of shiga-like toxin-producing Escherichia coli by multiplex PCR.

 

Adriana Liptakova1*, Hedviga Sehnalkova2, Marian Sabol1, Hana Kerestesova1, Jan Rosocha3, Daniela Kotulova4 & Leonard Siegfried1

1 Institute of Medical Microbiology, Faculty of Medicine, Pavol Jozef Safarik University, Srobarova 2, SK-04001, Kosice, Slovakia; tel.:++ 421 95 6225471, e-mail: aliptak@central.medic.upjs.sk

2 Department of Microbiology, Faculty Hospital Kosice, Slovakia

3 Associated Tissue Bank, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Slovakia

4 Microbiological Institute, Medical Faculty, Komensky University Bratislava, Slovakia

* corresponding author

Received: January 19, 2001 / Accepted: April 23, 2001

 

Abstract

Using multiplex PCR, we detected Escherichia coli strains harbouring genes for virulence factors typical for Shiga-like toxin-producing Escherichia coli strains. In our study we included two groups of patients: the first with uncomplicated diarrhoea and the second with clinical signs of haemolytic uremic syndrome. The aim of study was to find STEC among children with diarrhoea and haemolytic uremic syndrome, and to confirm the presence of STEC in those suffering from HUS using multiplex PCR. We have found STEC among children with gastroenteritis but we did not detect any STEC in children with HUS.

 

Key words: STEC, multiplex PCR, shiga-like toxins, HUS.