Biologia, Bratislava, 56/Suppl. 9: 55-59, 2001.
ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).
Incidence and transfer of antibiotic resistance in clinical isolates of Escherichia coli.
Marta Kmetova1*, Leonard Siegfried1, Marian Sabol1, Eva Bogyiova1, Zuzana Sandorcinova1, Anna Kerestesova1, Adriana Liptakova1 & Maria Molokacova2
1 Institute of Medical Microbiology, Faculty of Medicine, Pavol Jozef Safarik University, Srobarova 2, SK-04180 Kosice, Slovakia, e-mail: email@example.com
2 Department of Clinical Microbiology, University Hospital, Tr. SNP 1, SK-04066 Kosice, Slovakia
* corresponding author
Received: February 9, 2001 / Accepted: April 23, 2001
The authors present an incidence of resistance among 590 clinical Escherichia coli (E. coli) isolates to selected antibiotics and their capability to transfer resistance to recipient E. coli K-12 C 600 RifR strain. The lowest resistance (0.68%) was found against the third generation cephalosporins-ceftazidime, cefotaxime and also against netilmicin. Incidence of resistance increased in order from gentamicin (3.7%), ticarcilin (13.9%), trimethoprim/sulfamethoxazole (14.6%), chloramphenicol (15.3%), amoxicillin/clavulanic acid (17.1%), tetracycline (22%), streptomycin (28%) and ampicillin (46%). Transfer of resistance decreased in order from ceftazidime (75%), amoxicillin/clavulanic acid (61%), ampicillin (57%), gentamicin (50%), streptomycin (49%), tetracycline (39%), cotrimoxazol (20%), chloramphenicol (16%) and ticarcillin (11%). Plasmid DNA encoding antimicrobial resistance was analysed in 46 of E. coli isolates and their transconjugants. 23.3 kbp plasmid was found in 30 donor strains and the same plasmid was detected in 28 transconjugants (93%).
Key words: conjugation, antibiotic resistance transfer, Escherichia coli.