Biologia, Bratislava, 55/Suppl. 8: 103-106, 2000.
ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).
Effect of stobadine on superoxide generation and degranulation of stimulated human polymorphonuclear leukocytes in vitro.
Jana Pecivova1*, Tatiana Macickova1, Rado Nosal1 & Edita Danihelova2
1 Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dubravska cesta 9, SK-84216 Bratislava; e-mail: email@example.com
2 Institute for Haematology and Transfusiology, Partizanska 4, SK-83100 Bratislava, Slovakia
* corresponding author
Received: July 10, 2000 / Accepted: October 24, 2000
We studied the effect of stobadine, a pyridoindole antioxidant agent, on superoxide anion (O2.-) generation (respiratory burst) and enzyme (lysozyme and myeloperoxidase) release from FMLP (a specific receptor activator) and from PMA (activator of protein kinase C) stimulated human polymorphonuclear leukocytes (PMNL). Stobadine (1, 10 and 100 µM) decreased O2.- generation in FMLP stimulated PMNL only. It had no significant effect on enzyme release. The stobadine effect on O2.- generation seems to be linked to signal transduction rather than to its free radical scavenging and antioxidant properties since there was no effect on O2.- generation in PMA stimulated PMNL.
Key words: human PMN leukocytes, stobadine, superoxide anion, enzyme release, FMLP, PMA.