Biologia, Bratislava, 55/Suppl. 8: 69-73, 2000.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full Paper

Stobadine protects isoproterenol-induced toxic damage in rats.

 

Tatiana Macickova* & Jana Navarova

1Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dubravska cesta 9, SK-84216 Bratislava, Slovakia; e-mail: exfatama@savba.sk

* corresponding author

Received: July 10, 2000 / Accepted: October 24, 2000

 

Abstract

The pyridoindole stobadine (STO) is an effective cardioprotective drug with oxygen free radical scavenging properties. Isoproterenol (IPN), a synthetic catecholamine, is capable to induce massive myocardial necrosis accompanied with lysosomal enzyme (LE) activity changes in most mammals, when administered in high doses. The present study investigated the ability of STO to protect experimental animals against IPN-induced toxic damage. The activities of the lysosomal enzymes cathepsin D and N-acetyl--D-glucosaminidase were studied in the rat heart as markers of cell damage. Male wistar rats weighing 280-300g were used for these experiments. IPN-induced toxic damage in rats (9 h after administration, 50 mg.kg-1 s.c.) Was manifested by marked alterations in the activities of LE in the sedimentable fraction of the rat myocardium. STO administered in various dosage regimens reduced or eliminated the IPN-induced biochemical changes in the rat myocardium. From the results presented in this study we conclude that STO is able to protect rats against IPN-induced toxic damage.

 

Key words: stobadine, isoproterenol, lysosomal enzymes, cardioprotection, in vivo, rats.