Biologia, Bratislava, 55/Suppl. 8: 39-43, 2000.
ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).
Tumorogenic action of streptozotocin on the kidney in female newborn Wistar rats.
Stefan Galbavy1*, Ladislav Turecky2, Ladislav Zlatos3, Anna Gvozdjakova4
1 Institute of Pathological Anatomy, Medical Faculty, Comenius University, Sasinkova 4, SK-81108 Bratislava, Slovakia; fax: ++ 421 7 59357592
2 Institute of Biochemistry, Medical Faculty, Comenius University, Sasinkova 2, SK-81108 Bratislava, Slovakia
3 Institute of Pathological Physiology, Medical Faculty, Comenius University, Sasinkova 4, SK-81108 Bratislava, Slovakia
4 3rd Department of Internal Medicine, Comenius University, Limbova 5, SK-83308 Bratislava, Slovakia
* corresponding author
Received: July 10, 2000 / Accepted: October 24, 2000
Female newborn Wistar rats were used for the experiments. Animals were divided into two groups: controls and with diabetes mellitus. The rats were made diabetic by two intraperitoneal injections of streptozotocin at a dose of 45mg/kg weight on the 2nd and 9th day of life. Controls received an equivalent volume of citrate buffer. Animals were sacrificed at 7 months of age by decapitation. In the group of diabetic animals, kidney tumors were found in 8 of 10 animals. The tumors were unilateral and resembled microscopically nephroblastomas seen in man. The tumors were composed of spindle cells and epithelial glands. Both elements appeared to be malignant, though in the absence of metastases. This study demonstrated that streptozotocin in the total dose of 90 mg/kg body weight (given in two doses of 45 mg/kg within the first 9 days of life) had an exceedingly marked tumorogenic action on the kidney of female newborn rats, while it had no effect on the pancreas, nor did it induce distant metastases.
Key words: streptozotocin, Wistar rats, nephroblastoma, diabetes mellitus, histopathology.