5th Slovak – Czech Interdisciplinary Conference on Toxicology



1 – 3 June, 2000

Trenčianske Teplice, Slovakia



Reported by Eduard Ujházy, Jana Navarová & Mojmír Mach

Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia


The fifth Slovak-Czech interdisciplinary  toxicologic conference „Toxicology at the Threshhold of the Third Millenium“ was held in the Concert Hall of the pitoresque spa of Trenčianske Teplice from May 31 through June 3, 2000. The organizers were the Institute of Experimental Pharmacology of the Slovak Academy of Sciences, the Toxicology  Section of the Czech Society of Experimental and Clinical Pharmacology and Toxicology of the Czech Medical Association of J.E.Purkyně, the Institute of Preventive and Clinical Medicine,  the Faculty of Pharmacy and the Faculty of Philosophy of Comenius University, the Drug Research Institute in Modra, Inc., and the Slovak Association of Laboratory Animal Science. The principal sponsor was Slovakofarma, Inc., Hlohovec, under the auspices of its  Chief Executive Dipl. Ing. Ondřej Gattnar, PhD. The event was sponsored also by the Drug Research Institute, Inc., Modra, Ergon, Inc., and by Pharmacia & Upjohn, Bratislava. The company Bio-Chrom, Ltd., displayed its products at the Conference venue.

            The Conference was attended  by over 80 renown scientific workers from the Slovak and Czech Republics, engaged in the field of  experimental, clinical and industrial toxicology. A total of 38 papers were presented.

The scientific contributions presented at the Conference were particularly concerned with problems expected to be topical in the third millennium. Focus was on relative safety of drugs and xenobiotics from the viewpoint both of the human population and of whole ecosystems, as well as of a unified system of legislature concerning risk assessment of the environment.


The program of the Conference consisted of  six sections and a minisymposium:


1. Developmental Toxicology and Teratology

In the section concerning developmental toxicology and teratology, problems were discussed involving risk factors for the developing organisms as represented by drugs, chemical substances, stressogenic factors, oxygen reactive species, along with possibilities of prevention of their action and treatment of detrimental effects.


2. Organ Toxicity

The second session of the conference dealt with drug nephrotoxicity in man, interaction of the metals with cyanamide regarding their toxicity and toxicokinetics, role of polyamines in remodeling of pulmonary artery in hypoxic pulmonary hypertension. The following papers presented long term effect of streptozotocin induced diabetes mellitus in newborn rats on the kidney, renal pelvis changes after chronic administration of immunomodulatory substances, combination of dietary cholesterol access and gamma irradiation on mouse aorta, incidence of mammary tumors after 1-methyl-1-nitrosourea in mice, acute toxic effect of chloridazone on biochemical parameters and mitochondrial respiration.


3. Geno – Cyto – Mutagenicity

This section consisted of lectures concerning toxicokinetics of styrene and its metabolites in the evoluation of genotoxicity, the use of comet assay in in vitro testing of chemosensitivity in cancer patients. The potential antimutagenic effect of a-lipoic acid, comparison of several cationic amphiphilic drugs on phospholipase activity with their effect on stimulated human blood platelet aggregation, comparison of cadmium cytotoxicity in 79 cells after selenium pretreatment, phytoindication of genotoxic deteriorization of polluted environment in situ. 


4. Oxygen and Nitric Oxide

In this section papers were presented concerning the deleterious effects of reactive oxygen species on various tissues, organs and biological systems.


5. Legislature

Papers presented in the section Legislature dealt with regulations concerning preclinical assessment of medicinal products with special regard to cytostatics, the value of ecotoxicological tests in prediction of negative impacts on the ecosystems, the assessment program according to  OECD norms.


6. Miscellanea

The conference promoted mutual communication and exchange of scientific information not only in the problem area of toxicology proper but also in bordering disciplines such as pharmacology, endocrinology, molecular biology, animal physiology and magnetic resonance imaging method for visualizing of human as well as animal bodies.


  1. Interaction of Xenobiotics with Living Systems

The Minisymposium, coordinated by Dr.Vodička (Institute of Experimental Medicine, Czech Academy of Sciences, Prague) and by Dr. Štětina (Military Medical Academy JEP, Hradec Králové), was concerned with topical problems concerning the irreversible binding of (toxic) xenobiotics to blood proteins as well as to DNA. The focus was on the selection of potential markers of the damaged tissues.


The overall Conference topic was organically complemented by the Poster Section with 42 in vivo and in vitro experimental studies presented.


Abstracts of all the contributions were published in the Conference Proceedings in English. Selected papers are published in extenso in the present Supplement.


The participants and the Organizing Committee concluded this successful event by proclaiming to continue their intention to organize and participate in similar conferences annually, alternately in the Czech Republic and in the Slovakia. Hradec Králové in the Czech Republic was suggested as the conference venue and the date is to be September 2001.