Biologia, Bratislava, 55/Suppl. 8: 15-18, 2000.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full Paper

Effects of stobadine on hypoxia and hypoxia/reoxygenation injury in isolated hepatocytes from fasted rats.

 

Stefan Bezek* & Ivo Juranek

Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dubravska cesta 9, SK-84216 Bratislava, Slovakia; tel.: ++ 421 7 59410665, e-mail: exfasbez@nic.savba.sk

* corresponding author

Received: July 24, 2000 / Accepted: October 24, 2000

 

Abstract

Cell injury due to hypoxia and reoxygenation was investigated in  the system of parenchymal hepatocytes isolated from fasted rats. The functional and structural integrity of hepatocytes in hypoxia was evaluated on the basis of protein synthesis and [86Rb] release from prelabelled hepatocytes. Incubation of hepatocytes in nitrogen atmosphere resulted in significant inhibition of incorporation of [14C]-valine into  hepatocyte protein. Treatment of hepatocytes in hypoxia with the antioxidant stobadine (0.1, 1.0 and 10.0 mM)  reversed protein synthesis almost to control values. No changes were observed in [86Rb] release from prelabelled hepatocytes. In the experiment with hypoxia/reoxygenation, hepatocytes were exposed to 60 min hypoxia followed by 120 min reoxygenation and the injury was evaluated by lactate dehydrogenase (LD) leakage and  formation of thiobarbituric acid reactive substances (TBARS). The addition of stobadine at the given concentrations (0.1, 1.0 and 10.0 mM) before the onset of hypoxia resulted in dose dependent protection from hypoxia/reoxygenation injury of isolated hepatocytes.

 

Key words: hepatocytes, hypoxia/reoxygenation, antioxidan stobadine.