Biologia, Bratislava 54/Suppl. 6: 67-71, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full paper

Vacuolisation in the cerebellum after global brain ischaemia.


Ivo Vanicky*, Eniko Racekova & Jan Galik

Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4, SK-04001 Kosice, Slovakia; tel.: ++421 95 765 062, fax: ++421 95 765 074, e-mail:

* corresponding author

Received: February 18, 1999 / Accepted: October 5, 1999



This paper describes injury to the cerebellum following transient ischaemia induced by a high-pressure neck-tourniquet and systemic hypotension in the rat. In addition to a typical Purkinje cell necrosis, slowly evolving vacuolisation was observed in the neuropil of the deep nuclei of the cerebellum and in the cortical granular layer. We document that these vacuoles start to develop several hours after ischaemia and become maximally expanded after 24 hours. Then the vacuolisation gradually recedes and does not seem to be associated with neuronal loss. The possible mechanisms accounting for these alterations are discussed.


Key words: ischaemia, cerebellum, vacuolisation, deep cerebellar nuclei, rat.