Biologia, Bratislava 54/Suppl. 6: 183-188, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full paper

A comparative study of adrenergic innervation of the spleen in guinea-pigs and chickens.

 

Maria Sirotakova1*, Monika Kocisova2, Dusan Stopek1, Katarina Schmidtova2 & Frantisek Dorko2

1 Institute of Experimental Medicine, Medical Faculty, University P. J. Safarik, Tr. SNP 1, SK-04066 Kosice, Slovakia; tel.: ++421 95 6424606, fax: ++421 95 420253

2 Institute of Anatomy, Medical Faculty, University P. J. Safarik, Srobarova 2, SK- 04001 Kosice, Slovakia

* corresponding author

Received: December 17, 1998 / Accepted: October 5, 1999

 

Abstract

A comparative morphological study of adrenergic innervation of the spleen in guinea-pigs and chickens using the glyoxylic acid histofluorescence method has revealed that, in both cases, the adrenergic nerve components enter the spleen, accompanied with arteries and, in form of the plexiform perivascular bundles, they pass through the organ as far as their terminal parts. The thinner nerve branches and solitary varicose fibres, extending away from these perivascular plexuses, form a rich network lining in the peripheral side of the vascular media (so-called "adventitial plexus"); in guinea-pigs. They are also present in a remarkable number in fibrous trabeculae, in the marginal zone of periarteriolar lymphatic sheath (PALS), in the perifollicular topography and, sporadically, in sinuses and cords of the red pulp. The perifollicular nerve fibres in chickens are more abundantly represented than those in guinea-pigs. Innervation of fibrous capsule of the organ in almost the same in both animals although the fibrous trabecular system is insufficiently developed in chickens. In both cases, innervation of most of the venous trunks appears to be surprisingly abundant. The microscopic findings are consistent with the view that adrenergic nerve components in the spleen participate in the regulation of arterial motility as well as in the regulation of the microenvironment of the organís own parenchyma.

 

Key words: adrenergic innervation, spleen, guinea-pig, chicken.