Biologia, Bratislava 54/Suppl. 6: 203-207, 1999.
ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).
Visualization of rabbit microglial cells in the ischemic spinal cord segments by lectin method.
Institute of Neurobiology, Slovak Academy of Sciences, Soltesova 6, SK-04001 Kosice, Slovakia; tel.: ++421 95 765064, fax: ++421 95 765074, e-mail: email@example.com
Received: February 18, 1999 / Accepted: October 5, 1999
Histochemical method was used to visualize microglial cells in the lumbosacral spinal cord segments of the rabbit following a 20 min occlusion of the abdominal aorta. Microglia were visualized in vibratome sections using lectin staining with Griffonia simplicifolia B4-isolectin. Microglial staining was detected in all examined regions that were damaged by ischemia/reperfusion insult. The response of microglia was closely related to the neurological outcome at the end of post-ischemic reperfusion period (4 days). In the L3-S1 cord transverse sections of animals without neurological impairment (Grade 0), activated microglia intensely stained with GSA I-B4-HRP, were distributed homogeneously throughout the spinal cord white and gray matter. In the group of animals with incomplete neurological impairment (Grade 1), microglia were widespread with irregular distribution in the spinal gray matter and located, predominantly, in the ischemic areas of the ventral horn. Activated microglia clearly responded by mapping the different neuronal pools affected by the ischemic injury in the spinal cord gray matter. Our results reconfirm the applicability of the GSA I-B4-HRP method for the visualization of a microglial population in the rabbit spinal cord.
Key words: rabbit, spinal cord, ischemia, microglia, lectin.