Biologia, Bratislava 54/Suppl. 6: 215-220, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full paper

Substance-P-positive structures in the thoracic part of the rabbit’s medulla.


Silvia Rybarova*, Darina Kluchova, Monika Kocisova, Katarina Schmidtova, Maria Miklosova, Kvetuse Lovasova & Frantisek Dorko

Faculty of Medicine, P. J. Safarik University, Srobarova 2, SK-04001 Kosice, Slovakia; tel.: ++421 95 6228866, e-mail:

* corresponding author

Received: January 4, 1999 / Accepted: October 5, 1999



We used the modified indirect enzymatic immunohistochemical method to prove the presence of neuropeptide (NP) substance-P (SP) in the medulla of the Chinchilla rabbit (n=5). We observed that, similar to other animal species, the highest SP levels were found in the dorsal horns of the medullary grey matter, namely in their surface laminae (laminae I and II). The high occurrence of this SP was also observed in the adjacent zone of Lissauer, round the central channel (the so-called pericentral zone - lamina X) and in the place where the autonomous sympathetic nucleus nc. intermediolateralis (IML nucleus) is localised. The IML nucleus is located on the boundary of the grey and white medullary matter at, approximately, the level of lamina VII. The SP-positivity in the above mentioned zones appeared as strongly-stained dark-brown neurophils with nerve fibres clearly visible in some places. These fibres, mainly those in the deeper dorsal horn laminae, run singly in all directions. The presence of substance-P in the same zones of the rabbit medulla suggests that it has multiple functions in the organism. A significant role in the transfer of nociceptive information and the regulation of autonomous processes are amongst the most important of these functions.


Key words: rabbit, substance-P, medulla, immunohistochemistry.