Biologia, Bratislava 54/Suppl. 6: 189-196, 1999.
ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).
Efferent and afferent projections of the transplanted olfactory bulb: DiI study.
Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 6, SK-04001 Kosice, Slovakia; tel.: ++421 95 765064, fax: ++421 95 765074, e-mail: email@example.com
* corresponding author
Received: December 28, 1998 / Accepted: October 5, 1999
This study of projections of olfactory bulb transplants to higher olfactory centres was accomplished with fluorescent carbocyanine dye DiI applied in two different ways. After a unilateral olfactory bulbectomy in neonatal rats (3-5 days old), autoradiographically prelabelled, presumptive olfactory bulbs from E15-17 embryos were transplanted in place of the removed tissue. Three months after surgery the anaesthetized animals were perfusion fixed and a crystal of carbocyanine dye DiI was inserted into the putative transplant. In the second part of our investigation, DiI crystals were applied directly to the embryonic tissue at the time of transplantation. The appearance of DiI labelling differed depending on whether it was used in fixed tissue or in vivo. Both experimental paradigms resulted in the re-establishment of connections between the transplanted olfactory bulb and olfactory related structures in the host brain.
Key words: olfactory bulb, homotopic transplantation, connectivity, carbocyanine DiI.