Biologia, Bratislava 54/Suppl. 6: 129-137, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full paper

Newborn murine submandibular gland transplants within the brains of adult mice: a histochemical study of transplant cytodifferentiation, revascularization and reinnervation.


Blanka Pospisilova*, Olga Prochazkova, Dasa Slizová, Otakar Krs & Petr Bilek

Department of Anatomy, Charles University, Faculty of Medicine, Simkova 870, CZ-500 01 Hradec Kralové, Czech Republic; tel.: ++420 49 5816230, fax: ++420 49 5511711, e-mail:

* corresponding author

Received: January 8, 1999 / Accepted: October 5, 1999



Histochemical activities of alkaline phosphatase (AP), dipeptidylpeptidase (DPP) IV, esteroproteases (EP), succinate dehydrogenase (SDH), and acetylcholinesterase (AChE) were investigated in the developing parenchyma of intracerebral grafts of submandibular glands of newborn mice over the course of nine weeks and at five months following transplantation. Eight weeks after grafting, the enzyme pattern of the parenchyma of transplants was comparable with the enzyme pattern of a fully mature submandibular gland in situ. Histochemically positive granular convoluted tubules were present only in transplants that developed in the brains of male recipients. This finding reflects an androgenous dependence of granular convoluted tubules in the transplant experiment. A light microscopy examination showed that surviving submandibular gland transplants contained AP-positive capillaries, AChE-positive nerve fibers, and groups of cholinergic neurons.


Key words: submandibular gland, sexual dimorphism, transplant, graft, histochemistry.