Biologia, Bratislava 54/Suppl. 6: 61-66, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full paper

Phospholipid composition in the spinal cord regions after sublethal ischaemia followed by short- and long-lasting reperfusions.

 

Jaroslav Pavel* & Nadezda Lukacova

Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, SK-04001 Kosice, Slovakia; tel.: ++421 95 765064, fax: ++421 95 765074, e-mail: pavel@saske.sk

* corresponding author

Received: February 18, 1999 / Accepted: October 5, 1999

 

Abstract

The degradation of membrane lipids is an important factor in the pathogenesis of ischaemic damage and may reflect the extent of ischaemia/reperfusion injury of CNS. The aim of this study was to investigate the changes of membrane-bound phospholipids, sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatic acid (PA) and phosphatidylethanolamine (PE) in five spinal cord regions after sublethal (8 min) ischaemia followed by 1h or 24 h of reperfusion. Sublethal ischemia followed by 1h of reperfusion caused a significant degradation of SM in the dorsal horns and of SM, PC and PS in the intermediate zone. On the other hand, a significant increase of PI and PA concentrations was detected in the gray matter taken as a whole. The extent of damage of membrane-bound phospholipids in three gray matter regions varied in descending order: intermediate zone >dorsal horns>ventral horns. Sublethal ischaemia followed by 24 h of reperfusion increased the concentration of all phospholipids compared with the control group. However, the dorsal horns of the spinal cord were shown to be the site of a considerably enhanced resynthesis of phospholipids.

 

Key words: phospholipids, sublethal ischaemia, spinal cord regions, rabbit.