Biologia, Bratislava 54/Suppl. 6: 163-167, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full paper

Spinal cord white matter injury after single and repeated ischaemia/reperfusion observed by a light microscope.


Eva Mechirova1*, Ladislav Zacharias1, Pavol Jalc2 & Iveta Domorakova1

1 Department of Histology and Embryology, Medical Faculty of P. J. Safarik University, Srobarova 2, SK-04001 Kosice, Slovakia; tel.: ++421 95 6222606, e-mail:

2 Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 6, SK-04001 Kosice, Slovakia

* corresponding author

Received: February 18, 1999 / Accepted: October 5, 1999



Using a light microscope, changes in the white matter of the lumbar spinal cord segments were studied in rabbits subjected to 25 min of single ischaemia followed by 24 h recirculation and the results were compared with those after 8-8-9 min of repeated ischaemia followed by 3x1 h recirculation. Sections were stained by the pyronine-methyl green method and semi-thin sections were stained by cresyl violet. These sections revealed only negligible changes in the myelinated nerve fibres after a single 25 min ischaemia and 24 h recirculation. The degree of ischaemic injury was considerably increased when ischaemia was repeated three times at periods of 8-8-9 min and at 3x1 h of interischaemic recirculation intervals. The light microscopic observations revealed a pronounced degree of myelinolysis. The main histopathological changes visible after repeated ischaemia with a short interischaemic interval were large, irregular intramyelinic dilatations; the vesicular disruption of the myelin sheath; and conspicuous periaxonal edema accompanied by a peripheral displacement of axons within the myelinated nerve fibres.


Key words: myelinated nerve fibre, repeated ischaemia, rabbit, spinal cord.