Biologia, Bratislava 54/Suppl. 6: 115-119, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).

 

Full paper

The innervation of the bursa cloacalis.

 

Monika Kocisova1*, Maria Sirotakova2, Frantisek Dorko1, Katarina Schmidtova1, Silvia Rybarova1, Maria Miklosova1 & Darina Kluchova1

1 Department of Anatomy, Faculty of Medicine, P. J. Safarik University, Srobarova 2, SK-04001 Kosice, Slovakia; tel. : ++421 95 6228866

2 Institute of Experimental Medicine, Faculty of Medicine, P. J. Safarik University, Tr. SNP 1, SK-04066 Kosice, Slovakia

* corresponding author

Received: January 4, 1999 / Accepted: October 5, 1999

 

Abstract

In the present study we investigated the presence, distribution and topography of intrabursal morphological features of the adrenergic and ACHE and NADPH-d positive nerve structures in one month old ducks, chickens and pheasants. Authors have used a thiocholine method modified for the demonstration of ACHE-positive nerve fibres, a method using glyoxylic acid for the indication of adrenergic nervous structures and an enzymatic histochemical method for observation of NADPH-d positivity. The results obtained from these methods were: 1. In the bursa cloacalis the distribution, architectonic and topography of adrenergic, ACHE and NADPH-d positive nerve formation in ducks, chickens and pheasants were corresponding. Nerve fibres are particularly founded in perivascular localization or as solitary fibres running through the fibrous septa around the typical lymphatic follicles. 2. Nerve formations are denser in the submucous and muscular layer between bursa cloacalis and proctodeum. Nerve fibres in the germinal center of lymphatic follicles were not found. 3. ACHE and NADPH-d positive nerve fibres, with gangliocyts in peribursal fibrous tissue and submucous layer, were found to be more present in ducks than adrenergic nerve profiles. 4. The innervation of veins in ducks, chickens and pheasants is very poor. The differences observed between ducks, chickens and pheasants are, from a morphological point of view, mainly in terms of quantity of the marks.

 

Key words: ACHE-positive, NADPH-d positive, adrenergic, innervation, bursa cloacalis, ducks, chickens, pheasants.