Biologia, Bratislava 54/Suppl. 6: 93-101, 1999.

ISSN 0006-3088 (Biologia). ISSN 1335-6399 (Biologia. Section Cellular and Molecular Biology).


Full paper





 R.Mestril *** 





Institute of Neurobiology

Slovak Academy of Sciences

Soltesovej 6, Kosice



Dept. of Medicine, University of California, 

San Diego, 9500 Gilman Drive

CA, 92093, USA


Loyola University Medicine Center

The Cardiovascular Institute

2160 South First Avenue, Maywood, Il, USA


University of Veterinary Medicine, Komenskeho 73,

 040 01 Kosice, Slovakia


Anesthesiology Research Laboratory

 9500 Gilman Drive - 0818 CA, 92093, USA



D.Cizkova, DVM, PhD

Institute of Neurobiology

Slovak Academy of Sciences

Soltesovej 6, Kosice


tel. : +42-95-764 062, fax: +42-95-765 074,














Regional distribution of HSP72 in brain and spinal cord in transgenic rats..


DasaASA CiCzIZkovaA1* *,1*,   WolfgangOLFGANG  Dillmann22, **, RubinUBIN Mestril33***, MilanILAN CizCIZek44 &**** and MartinMARTIN   MarsSala5*****5. 

1I Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 6, SK-04001 Kosice,   

* Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 6, Kosice, Slovakia.

Slovakia; tel.:++421 -95- 764 062,   fax:++421- 95- 765 074,   email:

22**  D Departmentt. of Medicine, University of California, San Diego, 9500 Gilman Drive, CA, 92093,, USAA;

3*** 3L Loyola University Medicine Center, The Cardiovascular Institute, 2160 South    First Avenue, , Maywood, IlL, USA;.

44**** University of Veterinary Medicine, Komenskeeho 73, SK-040  01 Kossice, Slovakiaa;

55***** Anesthesiology Research Laboratory,  University of California, San Diego, , 9500 Gilman Drivee-0818, CA, 92093, USA

* corresponding author

Received: March 10, 1999 / Accepted: October 5, 1999





Previous studies   have demonstrated that overexpression of myocardial   heat shock protein (HSP720) in transgenic rats or mice   correlates with reduced infarct size and enhanced myocardial function, following cardiac ischemia. In the present study, using immunohistochemical technique and monoclonal HSP72 antibody we provide anatomical mapping of HSP72 in CNS in rats genetically modified to overexpress HSP72. In this strain of animals the highest expression of HSP72 was found in the following structures i) brain: nucleus caudatus, hippocampal CA1 region, cerebral cortex (layers V-VI), occasionaly also in Purkinje cells of cerebellum and in ii) spinal cord: superficial dorsal horn   (laminae II-IV), a-motoneurons, dorsal column; ascending pathways

 - fasciculus gracilis, fasciculus cuneatus and descending pathways - corticospinal tract. These results demonstrate the ability of adenoviral - HSPi construct to transfer and achieve permanent overexpression of inducible HSP72 in specific CNS regions. This regional specificity of HSP72 expression also suggests that this strain of animals represents a valuable experimental tool to define a potential protective role of HSP72 in a variety of pathological conditions such as ischemia, trauma or neuronal excitotoxicity.


Key words: Nneuronal, transgenic, HSP72, vulnerability, protection.