Biologia, Bratislava, 57/Suppl. 11: 247-251, 2002.

ISSN 0006-3088 (Biologia).

 

Review

Amylolytic enzymes produced by the yeast Saccharomycopsis fibuligera.

 

Eva Hostinova*

Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, SK-84551 Bratislava, Slovakia; tel.: ++ 421 2 5930 7443, fax: ++ 421 2 5930 7416, e-mail: umbihost@savba.sk

* corresponding author

Received: January 11, 2002 / Accepted: March 07, 2002

 

Abstract

Two strains of the food-born amylolytic yeast Saccharomycopsis fibuligera were selected from a broad spectrum of S. fibuligera strains found in culture collections of microorganisms. These were analysed with respect to production and characterisation of their amylolytic enzymes. S. fibuligera KZ represents a strain synthesizing an amylolytic complex composed of a-amylase, glucoamylase and a-glucosidase. S. fibuligera IFO 0111 represents a strain producing only one amylolytic enzyme -glucoamylase, with a property unique among yeast amylases, namely the ability to degrade raw starch. Information on molecular-genetic aspects and enzymatic behaviour of amylolytic enzymes produced by both strains is presented.

 

Key words: Saccharomycopsis fibuligera, a-amylase, glucoamylase, a-glucosidase, raw starch degradation.